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Figure 7 | Microbial Informatics and Experimentation

Figure 7

From: Large-scale experimental studies show unexpected amino acid effects on protein expression and solubility in vivo in E. coli

Figure 7

Segregation of amino acid parameters by predicted surface-exposure vs. burial. The fractional content of each amino acid was divided according to the localization predicted by the program PHD-PROF. Ordinal logistic regressions were calculated between all sequence parameters listed in Table 1 and E or S values in the Analysis Dataset as described above. Potentially redundant variables (e.g., a [total ala] = ae [exposed ala] + ab [buried ala]) were culled separately for expression and solubility as described in the Methods section. Signed -log(p) values are shown for the parameters that showed a significant correlation with either expression or solubility after parameter culling, using a Bonferroni-corrected p-value threshold of 0.0007.

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Microbial Informatics and Experimentation

ISSN: 2042-5783