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Figure 2 | Microbial Informatics and Experimentation

Figure 2

From: Large-scale experimental studies show unexpected amino acid effects on protein expression and solubility in vivo in E. coli

Figure 2

Influence of four amino acids on expression level and solubility score. Proteins in the Analysis Dataset presented in Figure 1 were divided into 10 bins based on their fractional content of a single amino acid. The plots show the fractions of proteins within each bin scoring either 0 (no expression or completely insoluble) or 5 (very high expression or solubility); the error bars represent 95% confidence intervals based on counting statistics. Plots are shown for lysine (panels A and B), arginine (panels C and D), leucine (panels E and F), and isoleucine (panels G and H). The analyses presented in these data panels, but none of the others in the main text, were performed exclusively on the fraction of each amino encoded by common codons in order to factor-out the influence of rare codons (which is explained below).

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Microbial Informatics and Experimentation

ISSN: 2042-5783