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Table 5 PSI-MI assignments to annotated experimental techniques.

From: Stringent response of Escherichia coli: revisiting the bibliome using literature mining

Techniques

Statistics over the corpus

Frequency per Decade

PSI-MI Class

PSI-MI id

PSI-MI name

Freq

Mean

Std

VMR

1970

1980

1990

2000

MI:0659 experimental feature detection

MI:0659

experimental feature detection*

55%

2.44

2.76

2

64%

65%

67%

67%

 

MI:0833

autoradiography

25%

1.65

1.4

1

29%

35%

22%

22%

 

MI:0113

western blot

21%

4.95

3.38

2.25

-

13%

18%

44%

 

MI:0074

mutation analysis

20%

3.34

3.24

3

14%

20%

27%

32%

 

MI:0114

x-ray crystallography

4%

1.63

1.46

1

-

-

2%

8%

 

MI:0811

insertion analysis

4%

1.14

0.38

0

-

-

7%

4%

MI:0045 experimental interaction detection

MI:0091

chromatography technology

50%

4.23

4.14

4

100%

85%

55%

64%

 

MI:0254

genetic interference

42%

2.68

2.35

2

-

28%

69%

64%

 

MI:0807

comigration in gel electrophoresis

37%

2.51

2.13

2

21%

48%

51%

49%

 

MI:0045

experimental interaction detection*

36%

3.1

3.12

3

14%

45%

47%

41%

 

MI:0808

comigration in sds page

27%

2.02

1.61

0.5

7%

23%

33%

29%

 

MI:0099

scintillation proximity assay

24%

1.94

1.65

1

64%

35%

20%

15%

 

MI:0051

fluorescence technology

16%

1.84

1.8

1

7%

20%

5%

22%

 

MI:0071

molecular sieving

15%

2.25

3.19

4.5

29%

13%

15%

13%

 

MI:0217

phosphorylation reaction

13%

2.72

3.74

4.5

7%

8%

16%

14%

 

MI:0415

enzymatic study

12%

1.96

2.11

4

7%

8%

11%

19%

 

MI:0008

array technology

10%

8.47

7.47

6.13

-

-

-

22%

 

MI:0928

filter trap assay

9%

2.24

2.47

2

36%

13%

4%

6%

 

MI:0004

affinity chromatography technology

8%

2.33

2.44

2

-

5%

5%

13%

 

MI:0428

imaging technique

7%

1.57

1.41

1

-

8%

4%

11%

 

MI:0047

far western blotting

6%

1.5

0.82

0

-

3%

7%

8%

 

MI:0435

protease assay

6%

3.92

4.01

5.33

-

5%

5%

8%

 

MI:0017

classical fluorescence spectroscopy

6%

1.08

0.29

0

-

-

5%

11%

 

MI:0089

protein array

6%

1.64

1.62

1

-

3%

2%

11%

 

MI:0029

cosedimentation through density gradient

5%

5.22

3.94

1.8

43%

10%

-

2%

 

MI:0040

electron microscopy

4%

2.57

1.31

0.5

-

10%

-

4%

 

MI:0676

tandem affinity purification

3%

3.8

5.18

8.33

-

-

-

7%

 

MI:0054

fluorescence-activated cell sorting

3%

5.8

4.6

3.2

-

-

4%

4%

 

MI:0413

electrophoretic mobility shift assay

3%

1.6

0.77

0

-

-

5%

2%

 

MI:0012

bioluminescence resonance energy transfer

2%

6.25

5.68

4.17

-

-

4%

2%

 

MI:0018

two hybrid

2%

2

1.41

0.5

-

-

-

7%

 

MI:0053

fluorescence polarization spectroscopy

2%

5

2.94

0.8

-

3%

-

2%

 

MI:0397

two hybrid array

2%

2

1.41

0.5

-

-

2%

2%

 

MI:0227

reverse phase chromatography

2%

3.25

2.87

1.33

-

-

5%

1%

 

MI:0226

ion exchange chromatography

1%

1

0

0

-

-

-

1%

 

MI:0031

protein cross-linking with a bifunctional reagent

1%

7

4

2.29

-

3%

-

1%

 

MI:0052

fluorescence correlation spectroscopy

1%

1

0

0

-

-

-

1%

 

MI:0416

fluorescence microscopy

1%

2.5

0.71

0

-

-

-

2%

 

MI:0016

circular dichroism

1%

1.5

0.71

0

-

-

-

2%

 

MI:0225

chromatin immunoprecipitation array

1%

1

0

0

-

-

-

1%

 

MI:0872

atomic force microscopy

1%

1

0

0

-

-

-

1%

 

MI:0049

filter binding

1%

1

0

0

-

3%

2%

-

 

MI:0426

light microscopy

1%

1

0

0

-

-

-

2%

MI:0661 experimental participant identification

MI:0088

primer specific pcr

40%

10.38

15.87

22.5

-

8%

29%

95%

 

MI:0080

partial dna sequence identification by hybridization

27%

3.75

3.47

3

14%

30%

29%

26%

 

MI:0078

nucleotide sequence identification

20%

1.77

1.26

1

-

15%

25%

22%

 

MI:0103

southern blot

14%

3.04

2.05

1.33

-

8%

15%

19%

 

MI:0929

nothern blot

8%

5.56

4.8

3.2

-

3%

11%

11%

 

MI:0421

identification by antibody

6%

1.82

1.51

1

-

8%

5%

6%

 

MI:0427

identification by mass spectrometry

5%

1.67

0.94

0

-

-

4%

8%

 

MI:0082

peptide massfingerprinting

2%

1.5

0.71

0

-

-

-

5%

 

MI:0093

protein sequence identification

1%

1

0

0

-

-

2%

 
 

MI:0411

enzyme linked immunosorbent assay

1%

4

2

1

-

-

2%

1%

MI:0346 experimental preparation

MI:0714

nucleic acid transduction

26%

2.22

2.82

2

14%

15%

31%

29%

 

MI:0715

nucleic acid conjugation

6%

1.73

1.41

1

7%

3%

5%

7%

 

MI:0308

electroporation

5%

1.89

1.56

1

-

-

2%

9%

 

MI:0343

cdna library

3%

1

0

0

-

-

-

6%

MI:0190 interaction type

MI:0194

cleavage reaction

1%

1

0

0

-

3%

-

-

MI:0116 feature type

MI:0373

dye label

5%

1.2

0.45

0

7%

8%

5%

4%

  1. Experimental techniques were evaluated considering the number of documents where these concepts were annotated and the number of annotations in the corpus. Moreover, the frequency of annotation of experimental techniques was also estimated for documents published in the four decades (from 1970 to 2009). Statistical measures are detailed in the Methods and Materials section.
  2. * These PSI-MI general classes were used to identify techniques that did not map into any particular technique within the class.